Mouse Anti-EGFR Recombinant Antibody (clone 7A7)

CAT#: PABW-088

Recombinant Mouse Antibody (7A7) is capable of binding to Human EGFR, expressed in Chinese Hamster Ovary cells (CHO). 7A7 recognizes a site on the EGFR extracellular domain that is not accessible in its most stable conformations, but that becomes exposed upon treatment with a tyrosine kinase inhibitor.

Published Data Tested Data Gene Expression
Figure 2 7A7 and 7A7 Fc Silent do not detect EGFR expression by flow cytometry. Figure 3 7A7 and 7A7 Fc Silent do not detect EGFR expression by Western blot. Figure 4 7A7 and 7A7 Fc Silent do not detect EGFR expression by immunofluorescence.
Figure 1 Anti-Human EGFR Antibody (PABW-088) in ELISA Figure 2 Anti-Human EGFR Antibody (PABW-088) in DB
Figure 1 IF staining of human cell line A-431 Figure 2 IF staining of human cell line U-251 MG Figure 3 Colon Figure 4 Liver Figure 5 Kidney Figure 6 Testis Figure 7 Placenta Figure 8 Lymph node Figure 9 RNA cell line category: Cell line enriched (A-431)

Specifications

  • Immunogen
  • Human epidermal growth factor receptor
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • 7A7
  • Applications
  • ELISA, WB

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The EGFR antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Immunofluorescence. Flow Cytometry: Cells were prepared for flow cytometry by washing with PBS and then FACs buffer (2% FBS in PBS). Cell suspensions (1x10^6cells) were stained with 7A7, 7A7 Fc Silent, cetuximab or AF1280 antibodies, and incubated with Alexa 488-conjugated-anti-mouse, anti-human or anti-goat secondary antibodies respectively. Immunofluorescence: Sections were then incubated with either 7A7, 7A7 Fc Silent, cetuximab or AF1280 antibody overnight at 4°C. After washing with PBS, sections were incubated with Alexa-Fluor-594 conjugated anti-mouse, anti-human or anti-goat secondary antibodies respectively. Western Blot: PVDF membranes were blocked with 2% BSA in PBS and incubated with 7A7, 7A7 Fc Silent, anti-mouse EGFR "AF1280" or β-tubulin primary antibodies and anti-mouse or anti-goat HRP secondary antibodies.

Target

  • Alternative Names
  • ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; EGFR vIII; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
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Breast cancer biomarkers at key points during disease progression
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Throughout the course of my research, I have utilized this antibody several times, and each time I have had reliable results. It is now an essential component of my research workflow.
Breast cancer biomarkers at key points during disease progression
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The thorough information on the product website made it easy to understand the antibody's usage and performance goals. I'm pleased that I made the purchase.
Breast cancer biomarkers at key points during disease progression
Reasonable price
This antibody is still reasonably priced without compromising on quality. Because it yields good outcomes on par with more expensive choices, it is a cost-effective solution.

Q&As

  1. What is the origin of the Mouse Anti-EGFR Recombinant Antibody (clone 7A7)?

    A: An epidermal growth factor receptor (EGFR)-targeting mouse monoclonal antibody is the source of the Mouse Anti-EGFR Recombinant Antibody (clone 7A7). Numerous cancer cell types frequently have excessive expression of this receptor.

  2. What is the purity level of this antibody?

    A: Mouse Anti-EGFR Recombinant Antibody (clone 7A7) is usually above 95% pure, as determined by SDS-PAGE, to guarantee the least amount of contaminating proteins and the highest level of specificity.

  3. Can this antibody detect low-abundance proteins?

    A: Despite its high affinity for EGFR, signal amplification methods or higher sample loading may be necessary to detect low-abundance proteins in order to obtain meaningful results.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABW-088, RRID: AB_3111682)

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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