Mouse Anti-EGFR Recombinant Antibody (clone 7A7)
CAT#: PABW-088
Recombinant Mouse Antibody (7A7) is capable of binding to Human EGFR, expressed in Chinese Hamster Ovary cells (CHO). 7A7 recognizes a site on the EGFR extracellular domain that is not accessible in its most stable conformations, but that becomes exposed upon treatment with a tyrosine kinase inhibitor.














Specifications
- Immunogen
- Human epidermal growth factor receptor
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- 7A7
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The EGFR antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Immunofluorescence. Flow Cytometry: Cells were prepared for flow cytometry by washing with PBS and then FACs buffer (2% FBS in PBS). Cell suspensions (1x10^6cells) were stained with 7A7, 7A7 Fc Silent, cetuximab or AF1280 antibodies, and incubated with Alexa 488-conjugated-anti-mouse, anti-human or anti-goat secondary antibodies respectively. Immunofluorescence: Sections were then incubated with either 7A7, 7A7 Fc Silent, cetuximab or AF1280 antibody overnight at 4°C. After washing with PBS, sections were incubated with Alexa-Fluor-594 conjugated anti-mouse, anti-human or anti-goat secondary antibodies respectively. Western Blot: PVDF membranes were blocked with 2% BSA in PBS and incubated with 7A7, 7A7 Fc Silent, anti-mouse EGFR "AF1280" or β-tubulin primary antibodies and anti-mouse or anti-goat HRP secondary antibodies.
Target
- Alternative Names
- ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; EGFR vIII; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- Gene ID
- 1956
- UniProt ID
- P00533
Customer Review
There are currently no Customer reviews or questions for PABW-088. Click the button above to contact us or submit your feedback about this product.



Q&As
-
What is the origin of the Mouse Anti-EGFR Recombinant Antibody (clone 7A7)?
A: An epidermal growth factor receptor (EGFR)-targeting mouse monoclonal antibody is the source of the Mouse Anti-EGFR Recombinant Antibody (clone 7A7). Numerous cancer cell types frequently have excessive expression of this receptor.
-
What is the purity level of this antibody?
A: Mouse Anti-EGFR Recombinant Antibody (clone 7A7) is usually above 95% pure, as determined by SDS-PAGE, to guarantee the least amount of contaminating proteins and the highest level of specificity.
-
Can this antibody detect low-abundance proteins?
A: Despite its high affinity for EGFR, signal amplification methods or higher sample loading may be necessary to detect low-abundance proteins in order to obtain meaningful results.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABW-088, RRID: AB_3111682)
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Related Signaling Pathways
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
See other products for "Clone 7A7"
- CAT
- Product Name
See other products for "EGFR"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-750 | Anti-EGFR/HER1 Recombinant Antibody (TAB-750) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG1 - kappa |
TAB-003 | Anti-Human EGFR Recombinant Antibody (Cetuximab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
TAB-272MZ | Human Anti-EGFR Recombinant Antibody (TAB-272MZ) | FuncS | Chimeric (mouse/human) IgG |
TAB-302MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
TAB-308MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-308MZ-F(E)) | ELISA, FC | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-228CL | Anti-Human EGFR Recombinant Antibody (ABT-806) | WB, IHC | Antibody |
TAB-297MZ | Anti-Human EGFR Recombinant Antibody (H225) | ELISA, WB | Humanized antibody |
TAB-298MZ | Anti-Human EGFR Recombinant Antibody (Hu225) | ELISA, WB | Humanized antibody |
TAB-300MZ | Human Anti-EGFR Recombinant Antibody (TAB-300MZ) | ELISA | Humanized IgG |
TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-459 | Human Anti-EGFR Recombinant Antibody (clone C225); scFv Fragment | FC | Human scFv |
PSBL-460 | Human Anti-EGFR Recombinant Antibody (clone h-R3); scFv Fragment | ELISA, WB, FuncS | Humanized scFv |
HPAB-0060-WJ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0060-WJ-S(P)) | ELISA, RIA, FC | Human scFv |
HPAB-2194LY-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2194LY-S(P)) | ELISA, WB | Mouse scFv |
HPAB-2195LY-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-2195LY-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-270MZ | Human Anti-EGFR Recombinant Antibody (TAB-270MZ) | ELISA | Human antibody |
TAB-278MZ | Human Anti-EGFR Recombinant Antibody (TAB-278MZ) | Cyt, ELISA, Inhib | Human IgG |
TAB-279MZ | Human Anti-EGFR Recombinant Antibody (TAB-279MZ) | Cyt, ELISA, Inhib | Human IgG |
TAB-282MZ | Human Anti-EGFR Recombinant Antibody (TAB-282MZ) | Cyt, FuncS, Inhib | Human IgG |
TAB-283MZ | Human Anti-EGFR Recombinant Antibody (TAB-283MZ) | Cyt, FuncS, Inhib | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-271MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-271MZ) | FuncS | Mouse IgG2b |
TAB-273MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-273MZ) | FC, FuncS, IHC, IP, Inhib | Mouse IgG |
TAB-275MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-275MZ) | Cyt, FuncS, Inhib | Mouse IgG |
TAB-276MZ | Anti-Human EGFR Recombinant Antibody (LA1) | Cyt, FuncS | |
TAB-277MZ | Anti-Human EGFR Recombinant Antibody (LA22) | Cyt, FuncS |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (NEUT-724CQ) | IF, FC, WB, IP, Neut | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1101 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) | IHC-P | Rabbit IgG |
MOR-4570 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) | ELISA | Rabbit IgG |
MOR-4571 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0202-CN | Human Anti-EGFR Recombinant Antibody (clone E10) | ELISA, WB | Human IgG |
HPAB-0044-FY | Human Anti-EGFR Recombinant Antibody (clone TGM10-1) | FC | Humanized IgG |
HPAB-0089-FY | Human Anti-EGFR Recombinant Antibody (clone MIL27-3) | FC | Human IgG |
HPAB-0119-FY | Human Anti-EGFR Recombinant Antibody (clone huML66) | Inhib | Human IgG |
HPAB-0122-FY | Mouse Anti-EGFR Recombinant Antibody (clone L2-H3) | ELISA, WB | Humanized IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-165 | Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-165) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-464CQ | Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-464CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-003 | Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-003) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-040 | Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-040) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
AFC-TAB-119 | Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-119) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-2194LY-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2194LY-F(E)) | ELISA, WB | Mouse Fab |
HPAB-2195LY-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2195LY-F(E)) | ELISA | Mouse Fab |
HPAB-2196LY-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2196LY-F(E)) | ELISA | Mouse Fab |
HPAB-2197LY-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2197LY-F(E)) | ELISA | Mouse Fab |
HPAB-2198LY-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2198LY-F(E)) | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-AP881-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP881-YC) | ELISA, FC, IP, FuncS | Llama VHH |
HPAB-AP882-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP882-YC) | ELISA | Llama VHH |
HPAB-AP883-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP883-YC) | ELISA | Llama VHH |
HPAB-AP884-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP884-YC) | ELISA | Llama VHH |
HPAB-AP885-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP885-YC) | ELISA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0424-XY84 | AbPlus™ Anti-EGFR Magnetic Beads (pSEX81-6) | IP, Protein Purification |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0924-YC32 | Mouse Anti-EGFR Recombinant Antibody (VS-0924-YC32) - Cancer Stem Cell Marker | IHC, WB | Mouse IgG1 |
VS-0924-YC35 | Rabbit Anti-EGFR Antibody (VS-0924-YC35) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-1024-XY177 | Mouse Anti-NHP EGFR Recombinant Antibody (clone 225) | IF, IP | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0125-FY28 | Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera | FC, Cyt | Human IgG1, scFv-Fc |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0225-XY102 | CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102) | FC | Mouse IgG1, kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0325-XY735 | Anti-EGFR Immunohistochemistry Kit | IHC | |
VS-0525-XY2183 | Anti-Mouse EGFR Immunohistochemistry Kit | IHC | |
VS-0525-XY2182 | Anti-Human EGFR Immunohistochemistry Kit | IHC |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0425-YC340 | Recombinant Anti-EGFR Vesicular Antibody, EV Displayed (VS-0425-YC340) | ELISA, FC, Neut, Cell-uptake |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0525-YC65 | Recombinant Anti-EGFR (AA 269-278 x AA 526-535) Biparatopic Antibody, Tandem scFv (Clone Pep 2 x Clone Pep 3) | FC | Tandem scFv |
VS-0525-YC66 | Recombinant Anti-EGFR (AA 582-591 x AA 606-614) Biparatopic Antibody, Tandem scFv (Clone Pep 4 x Clone Pep 1) | FC | Tandem scFv |
VS-0525-YC68 | Recombinant Anti-EGFR (AA 526-535 x AA 600-605) Biparatopic Antibody, Tandem scFv (Clone Pep 3 x Clone Pep 5) | FC | Tandem scFv |
VS-0525-YC213 | Recombinant Anti-EGFR (Domain II x Domain III) Biparatopic Antibody, Tandem scFv | ELISA, FC, IF, IHC, IP | Tandem scFv |
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, SPR, Inhib, FuncS
Application: ELISA, IHC, FC, WB, IF, IA
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: WB, ELISA, FC, IHC, IF, IP
Application: ELISA, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.